38.10 Homocysteine

38.10.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 10.317495 1 0.001 **
Homocysteine 1.609800 1 0.2 ns
Study 3.440566 1 0.06 ns
GA_samp 18.308313 1 <0.0001 ***
Homocysteine:Study 2.942559 1 0.09 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 3.8904562 1 0.05 ns
Homocysteine 0.5264440 1 0.5 ns
Study 1.3187370 1 0.3 ns
GA_samp 320.8313438 1 <0.0001 ***
Homocysteine:Study 0.1966252 1 0.7 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 9.9110678 1 0.002 **
Homocysteine 0.5427003 1 0.5 ns
Study 0.0000171 1 1.0 ns
GA_samp 104.9065657 1 <0.0001 ***
Homocysteine:Study 0.0067309 1 0.9 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Homocysteine 1 Inf 18.331 18.331 <0.0001 ***
3 Study 1 Inf 9.003 9.003 0.003 **
4 GA_samp 1 Inf 0.497 0.497 0.5 ns
2 Homocysteine:Study 1 Inf 7.177 7.177 0.007 **
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Homocysteine 1 Inf 20.968 20.968 <0.0001 ***
3 Study 1 Inf 13.031 13.031 0.0003 ***
4 GA_samp 1 Inf 0.153 0.153 0.7 ns
2 Homocysteine:Study 1 Inf 9.075 9.075 0.003 **
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Homocysteine 1 Inf 9.007 9.007 0.003 **
3 Study 1 Inf 12.012 12.012 0.0005 ***
4 GA_samp 1 Inf 0.764 0.764 0.4 ns
2 Homocysteine:Study 1 Inf 6.536 6.536 0.01 *

38.10.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 19.85 (7.23, 54.23) <0.0001
Homocysteine 0.96 (0.88, 1.05) 0.37
StudySTOP 1.13 (0.82, 1.55) 0.46
GA_samp 1.13 (1.07, 1.19) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 2.43 (1.37, 4.24) 0.002
Homocysteine 0.92 (0.87, 0.97) 0.003
StudySTOP 1.27 (1.07, 1.53) 0.007
GA_samp 1.33 (1.29, 1.38) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.18 (0.09, 0.38) <0.0001
Homocysteine 0.94 (0.87, 1.00) 0.07
StudySTOP 1.04 (0.82, 1.31) 0.75
GA_samp 1.23 (1.18, 1.28) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 204.81 (35.07, 1195.99) <0.0001
Homocysteine 0.58 (0.44, 0.75) <0.0001
StudySTOP 0.12 (0.03, 0.48) 0.003
GA_samp 1.03 (0.95, 1.11) 0.48
Homocysteine:StudySTOP 1.52 (1.12, 2.07) 0.007
HOMA-IR
Var Effect P
(Intercept) 98.06 (15.09, 637.29) <0.0001
Homocysteine 0.52 (0.39, 0.70) <0.0001
StudySTOP 0.06 (0.01, 0.28) 0.0003
GA_samp 1.02 (0.93, 1.11) 0.70
Homocysteine:StudySTOP 1.68 (1.20, 2.35) 0.003
Glucose (mmol/L)
Var Effect P
(Intercept) 12.11 (8.11, 18.09) <0.0001
Homocysteine 0.87 (0.79, 0.96) 0.004
StudySTOP 0.46 (0.30, 0.72) 0.0005
GA_samp 0.99 (0.97, 1.01) 0.38
Homocysteine:StudySTOP 1.14 (1.03, 1.26) 0.01

38.10.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Homocysteine concentration (μM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 71.18965 14.79000 53.53742 46.93395 107.9808
STOP 80.27600 13.22001 22.96009 57.09790 112.8629
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 57.78913 4.455741 118.90472 49.60675 67.32115
STOP 73.66759 3.016144 29.70947 67.75598 80.09499
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 1.710109 0.1783390 139.12443 1.391481 2.101698
STOP 1.777680 0.0898705 38.08152 1.604758 1.969235
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 20.53547 4.131751 Inf 13.84338 30.46260
STOP 18.30740 1.390771 Inf 15.77476 21.24665
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.178813 1.1487381 Inf 3.352903 7.999069
STOP 3.931310 0.3218741 Inf 3.348463 4.615609
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.554999 0.2329685 Inf 5.116651 6.030900
STOP 4.826860 0.0926674 Inf 4.648610 5.011945

38.10.4 Comparisons

Effect for every 1 unit increase in Homocysteine concentration (μM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) Homocysteine5.3 / Homocysteine4.3 SCOPE 0.96 (0.88, 1.05) 0.4
PRL (ng/ml) Homocysteine5.3 / Homocysteine4.3 STOP 0.96 (0.88, 1.05) 0.4
hPL (μg/ml) Homocysteine5.3 / Homocysteine4.3 SCOPE 0.92 (0.87, 0.97) 0.003
hPL (μg/ml) Homocysteine5.3 / Homocysteine4.3 STOP 0.92 (0.87, 0.97) 0.003
GH2 (ng/ml) Homocysteine5.3 / Homocysteine4.3 SCOPE 0.94 (0.87, 1.00) 0.07
GH2 (ng/ml) Homocysteine5.3 / Homocysteine4.3 STOP 0.94 (0.87, 1.00) 0.07
Insulin (uU/ml) Homocysteine5.3 / Homocysteine4.3 SCOPE 0.58 (0.44, 0.75) <0.0001
Insulin (uU/ml) Homocysteine5.3 / Homocysteine4.3 STOP 0.88 (0.75, 1.02) 0.10
HOMA-IR Homocysteine5.3 / Homocysteine4.3 SCOPE 0.52 (0.39, 0.70) <0.0001
HOMA-IR Homocysteine5.3 / Homocysteine4.3 STOP 0.87 (0.74, 1.03) 0.10
Glucose (mmol/L) Homocysteine5.3 / Homocysteine4.3 SCOPE 0.87 (0.79, 0.96) 0.004
Glucose (mmol/L) Homocysteine5.3 / Homocysteine4.3 STOP 0.99 (0.97, 1.01) 0.5

38.10.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

38.10.6 Plots